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Differential gene expression involved in oxidative stress response caused by triethylene glycol dimethacrylate

Paper ID Volume ID Publish Year Pages File Format Full-Text
9675 642 2008 11 PDF Available
Title
Differential gene expression involved in oxidative stress response caused by triethylene glycol dimethacrylate
Abstract

Triethylene glycol dimethacrylate (TEGDMA) is a comonomer that is released from dental resin-based materials into hydrophilic solvents. The compound reduces cell vitality, and causes genotoxicity in mammalian cells in vitro. Here, we used gene expression profiling, combined with pathway analysis tools, to identify the molecular events associated with TEGDMA cytotoxicity in human fibroblasts using Affymetrix HG-U133A 2.0 GeneChip arrays. Increased ROS production and a cell cycle delay caused by 3 mm TEGDMA after a 6 h exposure were related to a cell response at the transcriptional level. The predominant biological processes associated with the genes that were differentially expressed in untreated and treated cell cultures included oxidative stress, cellular growth, proliferation and morphology, cell death, gene expression as well as DNA replication and repair. The most significantly upregulated genes were GEM (17-fold), KLHL24, DDIT4, TGIF, DUSP5 and ATF3, which are all related to the regulation of the cell structure, stress response, and cell proliferation. TXNIP was the most downregulated transcript (five-fold), whose gene product regulates the cellular redox balance. The downregulation of NRG1, ASPM, FBXO5, and PLK2 is linked to the regulation of cell proliferation and cell structure. The underlying mechanisms of the up- and downregulation of genes seem to be activated by the production of ROS, and the related regulation of the cellular redox balance disturbed in the presence of TEGDMA appears to be of the utmost importance. The coordinated induction of genes coding for oxidative stress response and antioxidant proteins is a critical mechanism of protection against TEGDMA-induced cell damage.

Keywords
Dental resin; Dimethacrylate; TEGDMA; Oxidative stress; Gene expression; Affymetrix
First Page Preview
Differential gene expression involved in oxidative stress response caused by triethylene glycol dimethacrylate
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 10, April 2008, Pages 1377–1387
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering