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Biocompatibility of poly(d,l-lactide-co-glycolide) nanoparticles conjugated with alendronate

Paper ID Volume ID Publish Year Pages File Format Full-Text
9677 642 2008 12 PDF Available
Title
Biocompatibility of poly(d,l-lactide-co-glycolide) nanoparticles conjugated with alendronate
Abstract

Nanoparticles made of a conjugate of poly(d,l-lactide-co-glycolide) with alendronate (PLGA–ALE NPs), were prepared by emulsion/solvent evaporation technique. The conjugation yield, determined by MALDI TOF analysis, was 30–35%. PLGA–ALE NPs size, evaluated by photon correlation spectroscopy, was 198.7 ± 0.2 nm. Haemocompatibility studies using different concentrations of PLGA–ALE NPs did not show any significant effect on haemolysis, leukocyte number, platelet activation, APTT and complement consumption, in comparison with blood incubated with phosphate buffered saline (PBS). A significant reduction of the prothrombin activity was demonstrated after incubation with 560 μg/ml of PLGA–ALE NPs; a significant increase was observed at the highest dilutions. The viability of human umbilical vein endothelial cells and bone marrow stromal cells (BMSC), evaluated through the neutral red test, was not affected by PLGA–ALE NPs. There were no significant differences in cell-associated alkaline phosphatase between BMSC incubated with PLGA–ALE NP- and PBS-added media. These results demonstrated that PLGA–ALE NPs had an acceptable degree of blood compatibility and were not cytotoxic; therefore, they may be considered suitable for intravenous administration.

Keywords
Poly(lactic-co-glycolic acid) copolymer; Bisphosphonate; Nanoparticle; Drug delivery; Blood compatibility
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Biocompatibility of poly(d,l-lactide-co-glycolide) nanoparticles conjugated with alendronate
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 10, April 2008, Pages 1400–1411
Authors
, , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us