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Engineering of an elastic large muscular vessel wall with pulsatile stimulation in bioreactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
9683 642 2008 9 PDF Available
Title
Engineering of an elastic large muscular vessel wall with pulsatile stimulation in bioreactor
Abstract

Tissue engineering offers a new approach for the construction of vascular substitutes in vitro with proper mechanical properties. Although success has been made in the engineering of small blood vessels (<6 mm in diameter), it remains a challenge to engineer large vessels (>6 mm in diameter) due to their insufficient biomechanical property. In the current study, an elastic large vessel wall (6 mm in diameter) was engineered by loading a polyglycolic acid (PGA) unwoven fiber scaffold seeded with smooth muscle cells (SMCs) on a vessel reactor designed with dynamic culture conditions. SMCs were isolated from canine carotid artery and expanded before seeding on a PGA fiber mesh. The cell-seeded PGA mesh was then loaded on a vessel reactor and subjected to pulsatile stimuli. Grossly, an elastic vessel wall was formed after 8 weeks of dynamic engineering. Histological examination showed well-orientated smooth muscle cells and collagenous fibers in the group with dynamic culture. In addition, the phenotype of SMCs was confirmed by positive staining of smooth muscle α-actin and calponin. On the contrary, disorganized smooth muscle cells and collagenous fibers were observed in the group under static culture without stimuli. Furthermore, the engineered vessels under dynamic culture exhibited significant improvements on biomechanical property over the one from static culture. Our results indicate that the approach developed in the current work is efficient for large vessel engineering. This approach may also be suitable for the engineering of other tissues with muscular tubular structure.

Keywords
Vessel engineering; Smooth muscle cell; Polyglycolic acid; Vessel reactor
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Engineering of an elastic large muscular vessel wall with pulsatile stimulation in bioreactor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 10, April 2008, Pages 1464–1472
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us