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Sequential release of bioactive IGF-I and TGF-β1 from PLGA microsphere-based scaffolds

Paper ID Volume ID Publish Year Pages File Format Full-Text
9689 642 2008 8 PDF Available
Title
Sequential release of bioactive IGF-I and TGF-β1 from PLGA microsphere-based scaffolds
Abstract

Growth factors have become an important component for tissue engineering and regenerative medicine. Insulin-like growth factor-I (IGF-I) and transforming growth factor-beta1 (TGF-β1) in particular have great significance in cartilage tissue engineering. Here, we describe sequential release of IGF-I and TGF-β1 from modular designed poly(l,d-lactic-co-glycolic acid) (PLGA) scaffolds. Growth factors were encapsulated in PLGA microspheres using spontaneous emulsion, and in vitro release kinetics was characterized by ELISA. Incorporating BSA in the IGF-I formulations decreased the initial burst from 80% to 20%, while using uncapped PLGA rather than capped decreased the initial burst of TGF-β1 from 60% to 0% upon hydration. The bioactivity of released IGF-I and TGF-β1 was determined using MCF-7 proliferation assay and HT-2 inhibition assay, respectively. Both growth factors were released for up to 70 days in bioactive form. Scaffolds were fabricated by fusing bioactive IGF-I and TGF-β1 microspheres with dichloromethane vapor. Three scaffolds with tailored release kinetics were fabricated: IGF-I and TGF-β1 released continuously, TGF-β1 with IGF-I released sequentially after 10 days, and IGF-I with TGF-β1 released sequentially after 7 days. Scaffold swelling and degradation were characterized, indicating a peak swelling ratio of 4 after 7 days of incubation and showing 50% mass loss after 28 days, both consistent with scaffold release kinetics. The ability of these scaffolds to release IGF-I and TGF-β1 sequentially makes them very useful for cartilage tissue engineering applications.

Keywords
Bioactivity; Biodegradable; Controlled drug delivery; Growth factors; Microsphere; Scaffold
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Sequential release of bioactive IGF-I and TGF-β1 from PLGA microsphere-based scaffolds
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 10, April 2008, Pages 1518–1525
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us