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In vivo imaging of gene transfer to the respiratory tract

Paper ID Volume ID Publish Year Pages File Format Full-Text
9691 642 2008 8 PDF Available
Title
In vivo imaging of gene transfer to the respiratory tract
Abstract

Imaging of in vivo gene expression using luciferase expression in various organs has been used for several years. In contrast to other organs, in vivo imaging of the lung, particularly after non-viral gene transfer has not been extensively studied. The aim of this study was to address several questions: (1) Does in vivo light emission correlate with standard tissue homogenate-based luciferase detection in a dose-dependent manner? Recombinant Sendai virus (SeV) transduces airway epithelial cells very efficiently and was used to address this question, (2) Is the sensitivity of the assay sufficient to detect non-viral gene transfer? We treated mice with SeV-Lux vector using our standard “sniffing” protocol, a method that predominantly results in lung deposition. Dose-related in vivo light emission was visible in all animals. Importantly, there was a significant correlation (r > 0.90, p < 0.0001) between the in vivo and ex vivo assays in both the left and right lung. We next transfected the nasal epithelium via nasal perfusion or the lungs (“sniffing”) of mice with a luciferase plasmid (pCIKLux) complexed to the cationic lipid GL67 (n = 25–27/group) and imaged luciferase expression in vivo 24 h after transfection. Gene expression was detectable in both organs. Correlation between the in vivo and ex vivo assays was significant (r = 0.52, p < 0.005) in the left, but not the right lung. The correlation in the nose was weaker (r = 0.45, p < 0.05). To our knowledge these studies show for the first time that this non-invasive method of assessing pulmonary gene transfer is viable for evaluating non-viral gene transfer agents.

Keywords
Lung; Gene transfer; Lipid; In vivo test; Image analysis
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In vivo imaging of gene transfer to the respiratory tract
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 10, April 2008, Pages 1533–1540
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us