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Biohybrid thin films for measuring contractility in engineered cardiovascular muscle

Paper ID Volume ID Publish Year Pages File Format Full-Text
9715 643 2010 9 PDF Available
Title
Biohybrid thin films for measuring contractility in engineered cardiovascular muscle
Abstract

In vitro cardiovascular disease models need to recapitulate tissue-scale function in order to provide in vivo relevance. We have developed a new method for measuring the contractility of engineered cardiovascular smooth and striated muscle in vitro during electrical and pharmacological stimulation. We present a growth theory-based finite elasticity analysis for calculating the contractile stresses of a 2D anisotropic muscle tissue cultured on a flexible synthetic polymer thin film. Cardiac muscle engineered with neonatal rat ventricular myocytes and paced at 0.5 Hz generated stresses of 9.2 ± 3.5 kPa at peak systole, similar to measurements of the contractility of papillary muscle from adult rats. Vascular tissue engineered with human umbilical arterial smooth muscle cells maintained a basal contractile tone of 13.1 ± 2.1 kPa and generated another 5.1 ± 0.8 kPa when stimulated with endothelin-1. These data suggest that this method may be useful in assessing the efficacy and safety of pharmacological agents on cardiovascular tissue.

Keywords
Soft tissue biomechanics; tissue biomechanics; Cardiac tissue engineering; Cardiomyocyte; Smooth muscle cell; Mechanical properties
First Page Preview
Biohybrid thin films for measuring contractility in engineered cardiovascular muscle
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 13, May 2010, Pages 3613–3621
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering