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Bioengineering of a functional sheet of islet cells for the treatment of diabetes mellitus

Paper ID Volume ID Publish Year Pages File Format Full-Text
9735 644 2009 7 PDF Available
Title
Bioengineering of a functional sheet of islet cells for the treatment of diabetes mellitus
Abstract

The present study was designed to establish a novel tissue engineering approach for diabetes mellitus (DM) by fabricating a tissue sheet composed of pancreatic islet cells for in vivo transplantation. Pancreatic islet cell suspensions were obtained from Lewis rats, and plated onto temperature-responsive culture dishes coated with extracellular matrix (ECM) proteins. After the cells reached confluency, islet cells cultured on laminin-5 coated dishes were successfully harvested as a uniformly spread tissue sheet by lowering the culture temperature to 20 °C for 20 min. The functional activity of the islet cell sheets was confirmed by histological examination and Insulin secretion assay prior to in vivo transplantation. Histological examination revealed that the harvested islet cell sheet was comprised of insulin- (76%) and glucagon- (19%) positive cells, respectively. In vivo functionality of the islet cell sheet was maintained even 7 days after transplantation into the subcutaneous space of Lewis rats. The present study describes an approach to generate a functional sheet of pancreatic islet cells on laminin-5 coated temperature-responsive dishes, which can be subsequently transplanted in vivo. This study serves as the foundation for the creation of a novel cell-based therapy for DM to provide patients an alternative method.

Keywords
Islet transplantation; Cell sheet; Laminin-5; Monolayer culture
First Page Preview
Bioengineering of a functional sheet of islet cells for the treatment of diabetes mellitus
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 30, October 2009, Pages 5943–5949
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering