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The disruption of bacterial membrane integrity through ROS generation induced by nanohybrids of silver and clay

Paper ID Volume ID Publish Year Pages File Format Full-Text
9739 644 2009 9 PDF Available
Title
The disruption of bacterial membrane integrity through ROS generation induced by nanohybrids of silver and clay
Abstract

Nanohybrids, synthesized via silver nitrate reduction in the presence of silicate clay, exhibit a high potency against bacterial growth. The plate-like clay, due to its anionic surface charges and a large surface area, serves as the support for the formation of silver nanoparticles (AgNPs) ∼30 nm in diameter. The nanohybrid consisting of Ag/silicate at a 7/93 weight ratio inhibited the growth of dermal pathogens including Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa and Streptococcus pyrogens, as well as the methicillin- and oxacillin-resistant S. aureus (MRSA and ORSA). Scanning electron microscope revealed that these nanohybrids were adherent on the surface of individual bacteria. The thin silicate plates provide a surface for immobilizing AgNPs in one highly concentrated area but prevent them from entering the cell membrane. Subsequent cytotoxicity studies indicated that surface contact with the reduced AgNPs on clay is sufficient to initiate cell death. This toxicity is related to a loss in membrane integrity due to reactive oxygen species (ROS) generation. The hybridization of AgNPs on clay surface is viable for generating a new class of nanohybrids exhibiting mild cytotoxicity but high efficacy for battling drug-resistant bacteria.

Keywords
Silver nanoparticles; Reactive oxygen species; ClayAgNP, silver nanoparticle; PI, propidium iodide; CEC, cationic exchange capacity; SEM, scanning electronic microscope; FE-SEM, field emission scanning electron microscope; ICP-MS, inductively coupled plas
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The disruption of bacterial membrane integrity through ROS generation induced by nanohybrids of silver and clay
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 30, October 2009, Pages 5979–5987
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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