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The behavior of aged regenerated Bombyx mori silk fibroin solutions studied by 1H NMR and rheology

Paper ID Volume ID Publish Year Pages File Format Full-Text
9775 646 2008 7 PDF Available
Title
The behavior of aged regenerated Bombyx mori silk fibroin solutions studied by 1H NMR and rheology
Abstract

As part of a project to utilize the regenerated silk fibroin (RSF) membranes as a supporting matrix for the attachment and growth of corneal stem/progenitor cells in the development of tissue engineered constructs for the surgical restoration of the ocular surface, the behavior of the aged RSF solutions has been investigated. The solutions were produced from domesticated silkworm (Bombyx mori) cocoons according to a protocol involving successive dissolution steps, filtration and dialysis. The solutions were kept at 4 °C in a refrigerator for a certain period of time until near the gelation time. The changes in molecular conformation were studied by solution-state 1H NMR, while the flow of the solutions was characterized by rheological method. Upon ageing turbidity developed in solutions and the viscosity continuously decreased prior to a drastic increased near the gelation time. The 1H resonances of aged solutions showed a consistent downfield shift as compared to the 1H resonances of the fresh solution. Shear thinning with anomalous short recovery within a certain range of low shear rates occurred in both fresh and aged solutions. While the solutions behave as pseudo-plastic materials, the chain conformation in aged solutions adopted all secondary configurations with β-strand being predominant.

Keywords
Silk fibroin; Ageing; 1H NMR; Rheology; Chain conformation; Pseudo-plastic flow
First Page Preview
The behavior of aged regenerated Bombyx mori silk fibroin solutions studied by 1H NMR and rheology
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 32, November 2008, Pages 4268–4274
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering