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The mechanically enhanced phase separation of sprayed polyurethane scaffolds and their effect on the alignment of fibroblasts

Paper ID Volume ID Publish Year Pages File Format Full-Text
9815 648 2010 7 PDF Available
Title
The mechanically enhanced phase separation of sprayed polyurethane scaffolds and their effect on the alignment of fibroblasts
Abstract

This paper reports a method to fabricate anisotropic scaffolds of tunable porosity and mechanical properties. Scaffolds were fabricated using a computer controlled sprayed phase separation technique. Following fabrication, the sheets were elongated 0, 35 or 70% of their original length to induce varying degrees of scaffold alignment and anisotropy. The nonsolvent used in the phase separation was shown to affect porosity and the elastic modulus. Mouse embryo NIH-3T3 fibroblasts were cultured on the scaffolds to investigate cell response to the anisotropy of the scaffold. A 2D FFT method was used to quantify cellular alignment. Cells were shown to align themselves with the scaffold. This sheet-like scaffold material can be used in single plys or can be laminated to form porous 3D composite scaffolds.

Keywords
Scaffold; Cell alignment; Mechanical properties; Porosity; Polyurethane; Tissue engineering
First Page Preview
The mechanically enhanced phase separation of sprayed polyurethane scaffolds and their effect on the alignment of fibroblasts
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 6, February 2010, Pages 1126–1132
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering