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The repair of large segmental bone defects in the rabbit with vascularized tissue engineered bone

Paper ID Volume ID Publish Year Pages File Format Full-Text
9820 648 2010 9 PDF Available
Title
The repair of large segmental bone defects in the rabbit with vascularized tissue engineered bone
Abstract

Management of segmental bone defects is a considerable challenge for orthopedic surgeons. Tissue engineering is a promising method for repairing bone defects, and vascularization is critical to the performance of a tissue engineered bone. We report herein the construction of a vascularized tissue engineered bone with mesenchymal stem cells (MSCs) and MSC-derived endothelial cells (ECs) co-cultured in porous β-tricalcium phosphate ceramic (β-TCP) to repair 1.5-cm ulnar defects in the rabbit. Examination by X-ray and single photon emission computed tomography (SPECT), histologic analysis, and biomechanical tests were used to evaluate repair and the vascularization of the implants. The results showed that by co-seeding MSCs and MSC-derived ECs, the resulting vascularization was able to promote osteogenesis and improve mechanical properties. The rabbits treated with vascularized tissue engineered bone exhibited far more extensive osteogenesis and good vascularization. Therefore, we suggest that the vascularized tissue engineered bone constructed by co-culture of MSCs and MSC-derived ECs in porous β-TCP may be an effective approach to promote repair of segmental bone defects and have potential for repairing large segmental bone defects in a clinical setting.

Keywords
Bone tissue engineering; Mesenchymal stem cells; Endothelial cells; Bone repair; Vascularization
First Page Preview
The repair of large segmental bone defects in the rabbit with vascularized tissue engineered bone
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 6, February 2010, Pages 1171–1179
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering