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Glutathione-mediated release of functional plasmid DNA from positively charged quantum dots

Paper ID Volume ID Publish Year Pages File Format Full-Text
9864 650 2008 7 PDF Available
Title
Glutathione-mediated release of functional plasmid DNA from positively charged quantum dots
Abstract

DNA was efficiently bound to water-soluble positively charged CdTe quantum dots (QDs) through complementary electrostatic interaction. These QDs–DNA complexes were disrupted and DNA was released by glutathione (GSH) at intracellular concentrations. Interestingly, there was almost no detectable DNA released by extracellular concentration of GSH. The formation of QDs–DNA complexes and GSH-mediated DNA release from the complexes were confirmed by dye displacement assay, electrophoretic mobility shift assay (EMSA), transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS) experiments. The released DNA retained transcriptional activity and expressed enhanced green fluorescent protein (EGFP) after being transfected into HEK 293 cells. The transfection efficiency measured by flow cytometry (FCM) was comparable with the positive control. The obvious difference of GSH concentration in nature between the intra- and extracellular environments as well as the GSH concentration-dependent triggered release suggests potential applications of these positively QDs in selective unpacking of payload in living cells in a visible manner.

Keywords
QDs; Glutathione; Plasmid DNA; Controlled release
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Glutathione-mediated release of functional plasmid DNA from positively charged quantum dots
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 18, June 2008, Pages 2776–2782
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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