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A sandwich structured SiO2/cytochrome c/SiO2 on a boron-doped diamond film electrode as an electrochemical nitrite biosensor

Paper ID Volume ID Publish Year Pages File Format Full-Text
9866 650 2008 8 PDF Available
Title
A sandwich structured SiO2/cytochrome c/SiO2 on a boron-doped diamond film electrode as an electrochemical nitrite biosensor
Abstract

A novel sandwich structured SiO2 gel/cytochrome c (Cyt c)/SiO2 gel was designed and constructed on conductive boron-doped diamond (BDD) film substrate. A SiO2 gel membrane was first in situ deposited on the pretreated positive charged H-terminated BDD electrode with a simple and artful surface vapor sol–gel method. Cyt c was subsequently immobilized onto the SiO2 membranes by electrostatic attraction, followed by another SiO2 gel layer in situ depositing on it. The SiO2 interlayer was conceived to play an important role in the resultant sandwich structured SiO2/Cyt c/SiO2/BDD electrode as a selective “semi-open” medium, which guaranteed the immobilized Cyt c to maintain high stability and perform good electrochemistry and biocatalysis responses. The bioactivity of Cyt c was well protected and the immobilized biomolecule even didn't denature at extremely high or low pH condition. More attractively, Cyt c in the sandwich structured electrode could be further oxidized into highly reactive Cyt c π-cation by two-step electrochemical oxidation, which could oxidize NO2− into NO3− in the solution. A sensitive determination approach of nitrite was accordingly built up based on this biocatalytic oxidative interaction for the first time and a possible mechanism of the interaction was herein proposed.

Keywords
Sandwich structured; Protein; Bioelectrocatalysis; Diamond; Nitrite
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A sandwich structured SiO2/cytochrome c/SiO2 on a boron-doped diamond film electrode as an electrochemical nitrite biosensor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 18, June 2008, Pages 2794–2801
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us