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Identification, recombinant production and structural characterization of four silk proteins from the Asiatic honeybee Apis cerana

Paper ID Volume ID Publish Year Pages File Format Full-Text
9869 650 2008 9 PDF Available
Title
Identification, recombinant production and structural characterization of four silk proteins from the Asiatic honeybee Apis cerana
Abstract

Unlike silkworm and spider silks assembled from very large and repetitive fibrous proteins, the bee and ant silks were recently demonstrated to consist of four small and non-repetitive coiled-coil proteins. The design principle for this silk family remains largely unknown and so far no structural study is available on them in solution. The present study aimed to identify, express and characterize the Asiatic honeybee silk proteins using DLS, CD and NMR spectroscopy. Consequently, (1) four silk proteins are identified, with ∼6, 10, 9 and 8% variations, respectively, from their European honeybee homologs. Strikingly, their recombinant forms can be produced in Escherichia coil with yields of 10–60 mg/l. (2) Despite containing ∼65% coiled-coil sequences, four proteins have very low α-helix (9–27%) but unusually high random coil (45–56%) contents. Surprisingly, β-sheet is also detected in four silk proteins (26–35%), implying the possible presence of β-sheet in the bee and ant silks. (3) Four proteins lacking of the tight tertiary packing appear capable of interacting with each other weakly but this interaction triggers no significant formation of the tight tertiary packing. The study not only implies the promising potential to produce recombinant honeybee silk proteins for the development of various biomaterials; but also provides the first structural insight into the molecular mechanism underlying the formation of the coiled-coil silks.

Keywords
Asiatic honeybee Apis cerana; Silk protein; Coiled-coil; Recombinant biotechnology; Dynamic light scattering; Circular dichroism
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Identification, recombinant production and structural characterization of four silk proteins from the Asiatic honeybee Apis cerana
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 18, June 2008, Pages 2820–2828
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us