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Protein direct delivery to dendritic cells using nanoparticles based on amphiphilic poly(amino acid) derivatives

Paper ID Volume ID Publish Year Pages File Format Full-Text
9895 652 2007 10 PDF Available
Title
Protein direct delivery to dendritic cells using nanoparticles based on amphiphilic poly(amino acid) derivatives
Abstract

Induction of an adaptive immune response by vaccination is possible for a broad range of infectious diseases or cancers. Antigen-loaded polymeric nanoparticles have recently been shown to possess significant potential as vaccine delivery systems and adjuvants. Here we demonstrate the use of nanoparticles composed of amphiphilic poly(amino acid) derivatives as vaccine adjuvants. We prepared protein-loaded, biodegradable nanoparticles composed of hydrophobically modified poly(γ-glutamic acid) (γ-PGA). γ-PGA hydrophobic derivatives (γ-hPGA) formed 200 nm-sized nanoparticles in water. The protein-encapsulated γ-hPGA nanoparticles were efficiently taken up by immature dendritic cells (iDCs). Interestingly, the nanoparticle uptake by iDCs induced DC maturation. The immunization with human immunodeficiency virus (HIV)-1 gp120-encapsulated nanoparticles strongly induced antigen-specific cellular immunity. These results suggest that antigen-loaded γ-hPGA nanoparticles provide a novel delivery tool for vaccination against viral infections or tumors. This system has potential application as a universal delivery system for protein-based vaccines capable of inducing cytotoxic T lymphocyte (CTL).

Keywords
Nanoparticles; Vaccine; Protein delivery; Dendritic cells; Poly(γ-glutamic acid)
First Page Preview
Protein direct delivery to dendritic cells using nanoparticles based on amphiphilic poly(amino acid) derivatives
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 23, August 2007, Pages 3427–3436
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering