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Biomimetic composite coating on rapid prototyped scaffolds for bone tissue engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
990 70 2011 12 PDF Available
Title
Biomimetic composite coating on rapid prototyped scaffolds for bone tissue engineering
Abstract

The objective of this present study was to improve the functional performance of rapid prototyped scaffolds for bone tissue engineering through biomimetic composite coating. Rapid prototyped poly(ε-caprolactone)/tri-calcium phosphate (PCL/TCP) scaffolds were fabricated using the screw extrusion system (SES). The fabricated PCL/TCP scaffolds were coated with a carbonated hydroxyapatite (CHA)–gelatin composite via biomimetic co-precipitation. The structure of the prepared CHA–gelatin composite coating was studied by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. Compressive mechanical testing revealed that the coating process did not have any detrimental effect on the mechanical properties of the scaffolds. The cell–scaffold interaction was studied by culturing porcine bone marrow stromal cells (BMSCs) on the scaffolds and assessing the proliferation and bone-related gene and protein expression capabilities of the cells. Confocal laser microscopy and SEM images of the cell–scaffold constructs showed a uniformly distributed cell sheet and accumulation of extracellular matrix in the interior of CHA–gelatin composite-coated PCL/TCP scaffolds. The proliferation rate of BMSCs on CHA–gelatin composite-coated PCL/TCP scaffolds was about 2.3 and 1.7 times higher than that on PCL/TCP scaffolds and CHA-coated PCL/TCP scaffolds, respectively, by day 10. Furthermore, reverse transcription polymerase chain reaction and Western blot analysis revealed that CHA–gelatin composite-coated PCL/TCP scaffolds stimulate osteogenic differentiation of BMSCs the most, compared with PCL/TCP scaffolds and CHA-coated PCL/TCP scaffolds. These results demonstrate that CHA–gelatin composite-coated rapid prototyped PCL/TCP scaffolds are promising for bone tissue engineering.

Keywords
Rapid prototyping scaffolds; Biomimetic composite coating; Carbonated hydroxyapatite–gelatin composite; Bone tissue engineering
First Page Preview
Biomimetic composite coating on rapid prototyped scaffolds for bone tissue engineering
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 2, February 2011, Pages 809–820
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering