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The stimulation of angiogenesis and collagen deposition by copper

Paper ID Volume ID Publish Year Pages File Format Full-Text
9904 653 2010 8 PDF Available
Title
The stimulation of angiogenesis and collagen deposition by copper
Abstract

Copper is known to trigger endothelial cells towards angiogenesis. Different approaches have been investigated to develop vascularisation in biomaterials. The angiogenic and healing potential of copper ions in combination with two major angiogenic factors was examined. A 3D culture system in which, under stimulation by FGF-2 and to a lesser degree with VEGF, endothelial cells assembled into structures resembling to an angiogenic process was used. The combination of CuSO4 with increasing doses of VEGF or FGF-2 enhanced the complexity of angiogenic networks in a significant manner. In vivo studies were also conducted by incorporating FGF-2 with CuSO4 in a cylindrical collagen-based scaffold. CuSO4 enhanced significantly the invasion of microvessel compared to control implants and to 20 ng FGF-2 ± CuSO4. Vascular infiltration was also significantly improved by combination of CuSO4 with FGF-2, compared to FGF-2 alone (0.2 and 1 μg). Nevertheless, in comparison with CuSO4 alone, there was a significant increase only with 1 μg of FGF-2 combined with CuSO4. Significantly, collagen fiber deposition was enhanced following the combinatory loading in comparison to that with FGF-2 alone but not with CuSO4 only. Thus, copper associated with growth factors may have synergistic effects which are highly attractive in the fields of tissue engineering (e.g., bone) and biomaterials.

Keywords
Collagen scaffold; Angiogenesis; Growth factor; Copper; 3D angiogenesis assay; Collagen deposition
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The stimulation of angiogenesis and collagen deposition by copper
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 5, February 2010, Pages 824–831
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us