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Fatigue and human umbilical cord stem cell seeding characteristics of calcium phosphate–chitosan–biodegradable fiber scaffolds

Paper ID Volume ID Publish Year Pages File Format Full-Text
9906 653 2010 8 PDF Available
Title
Fatigue and human umbilical cord stem cell seeding characteristics of calcium phosphate–chitosan–biodegradable fiber scaffolds
Abstract

Calcium phosphate cement (CPC) has in situ-setting ability and bioactivity, but the brittleness and low strength limit CPC to only non-load-bearing bone repairs. Human umbilical cord mesenchymal stem cells (hUCMSCs) can be harvested without an invasive procedure required for the commonly studied bone marrow MSCs. However, little has been reported on hUCMSC delivery via bioactive scaffolds for bone tissue engineering. The objectives of this study were to develop CPC scaffolds with improved resistance to fatigue and fracture, and to investigate hUCMSC delivery for bone tissue engineering. In fast fracture, CPC with 15% chitosan and 20% polyglactin fibers (CPC–chitosan–fiber scaffold) had flexural strength of 26 mPa, higher than 10 mPa for CPC control (p < 0.05). In cyclic loading, CPC–chitosan–fiber specimens that survived 2 × 106 cycles had the maximum stress of 10 MPa, compared to 5 MPa of CPC control. CPC–chitosan–fiber specimens that failed after multiple cycles had a mean stress-to-failure of 9 MPa, compared to 5.8 MPa for CPC control (p < 0.05). hUCMSCs showed excellent viability when seeded on CPC and CPC–chitosan–fiber scaffolds. The percentage of live cells reached 96–99%. Cell density was about 300 cells/mm2 at day 1; it proliferated to 700 cells/mm2 at day 4. Wst-1 assay showed that the stronger CPC–chitosan–fiber scaffold had hUCMSC viability that matched the CPC control (p > 0.1). In summary, this study showed that chitosan and polyglactin fibers substantially increased the fatigue resistance of CPC, and that hUCMSCs had excellent proliferation and viability on the scaffolds.

Keywords
Calcium phosphate cement; Fatigue; Load-bearing; Bioactive scaffolds; Human umbilical cord stem cells; Bone regeneration
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Fatigue and human umbilical cord stem cell seeding characteristics of calcium phosphate–chitosan–biodegradable fiber scaffolds
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 5, February 2010, Pages 840–847
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us