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Creation of a long-lifespan ciliated epithelial tissue structure using a 3D collagen scaffold

Paper ID Volume ID Publish Year Pages File Format Full-Text
9907 653 2010 6 PDF Available
Title
Creation of a long-lifespan ciliated epithelial tissue structure using a 3D collagen scaffold
Abstract

We describe a method of using a 3D collagen gel scaffold applied at the air–liquid interface to culture dissociated primary tracheal-bronchial ciliated cells into a ciliated epithelial tissue structure (CETS). This 3D collagen gel culture system enables the induction of ciliogenesis and continuously provides support, maintenance, development, differentiation and propagation for the growth of cilia into the CETS. The CETS developed by this system resembles the ciliary metachronal motility and morphological, histological and physiopharmacological characteristics of cells found in native and in vivo ciliated epithelia. The CETS can be sustained for months with a straightforward and simple maintenance protocol. The integrity of the functional ciliary activity of this CETS enables the evaluation of long-term effects of many pulmonary drug candidates without using animals.

Keywords
Ciliated epithelial tissue structure; Ciliogenesis; 3D gel scaffold; Collagen
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Creation of a long-lifespan ciliated epithelial tissue structure using a 3D collagen scaffold
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 5, February 2010, Pages 848–853
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us