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Toxicity and imaging of multi-walled carbon nanotubes in human macrophage cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
9939 654 2009 9 PDF Available
Title
Toxicity and imaging of multi-walled carbon nanotubes in human macrophage cells
Abstract

Multi-walled carbon nanotubes (MWNTs) have been proposed for use in many applications and concerns about their potential effect on human health have led to the interest in understanding the interactions between MWNTs and human cells. One important technique is the visualisation of the intracellular distribution of MWNTs. We exposed human macrophage cells to unpurified MWNTs and found that a decrease in cell viability was correlated with uptake of MWNTs due to mainly necrosis. Cells treated with purified MWNTs and the main contaminant Fe2O3 itself yielded toxicity only from the nanotubes and not from the Fe2O3. We used 3-D dark-field scanning transmission electron microscopy (DF-STEM) tomography of freeze-dried whole cells as well as confocal and scanning electron microscopy (SEM) to image the cellular uptake and distribution of unpurified MWNTs. We observed that unpurified MWNTs entered the cell both actively and passively frequently inserting through the plasma membrane into the cytoplasm and the nucleus. These suggest that MWNTs may cause incomplete phagocytosis or mechanically pierce through the plasma membrane and result in oxidative stress and cell death.

Keywords
Multi-walled carbon nanotubes; Iron contaminant; Active passive uptake; Electron microscopy; Tomography; Confocal microscopy
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Toxicity and imaging of multi-walled carbon nanotubes in human macrophage cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 25, September 2009, Pages 4152–4160
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us