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Plasma-assisted surface chemical patterning for single-cell culture

Paper ID Volume ID Publish Year Pages File Format Full-Text
9945 654 2009 8 PDF Available
Title
Plasma-assisted surface chemical patterning for single-cell culture
Abstract

Polyethylene glycol (PEG)-like films were grafted onto various substrates by plasma polymerization of diethylene glycol dimethyl ether (diglyme). Low-power diglyme plasma conditions yielded PEG-like films exhibiting nonfouling behavior, as evidenced by the significant decrease in protein adsorption and cell attachment on substrates coated by these films. Different chemical patterns were produced on substrates with grafted PEG-like films using silicon or polydimethylsiloxane membrane shadow masks fabricated by photolithography and a simple one-step plasma etch process. Culture of single human mesenchymal stem cells on chemically patterned polystyrene dishes demonstrated a strong dependence of the actin structure and nuclear morphology on the cell shape and spreading area.

Keywords
Cell culture and morphology; Mesenchymal stem cells; Plasma polymerization; Polyethylene glycol-like film; Surface micropatterning
First Page Preview
Plasma-assisted surface chemical patterning for single-cell culture
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 25, September 2009, Pages 4203–4210
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering