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Alginate-based microencapsulation of retinal pigment epithelial cell line for cell therapy

Paper ID Volume ID Publish Year Pages File Format Full-Text
9961 655 2008 8 PDF Available
Title
Alginate-based microencapsulation of retinal pigment epithelial cell line for cell therapy
Abstract

The goals of this study were to evaluate human retinal pigment epithelial cell line (ARPE-19) for cell encapsulation and to optimize the alginate-based microencapsulation. We used immortalized ARPE-19 cells and the transfected sub-line that expresses secreted alkaline phosphatase (SEAP) reporter enzyme. Alginate was cross-linked with different divalent cations (Ca2+, Ba2+, Sr2+ and combination of Ca2+ and Ba2+), coated first with poly-l-lysine (PLL), and then with alginate. Microcapsules with different pore sizes and stability were generated. The pore size of the microcapsules was assessed by the release of encapsulated fluorescein isothiocyanate (FITC)-dextrans. The viability of the cells in the microcapsules was studied in vitro by assessing the secretion rates of SEAP and oxygen consumption by the cells. The best microcapsule morphology, durability and cellular viability were obtained with alginate microcapsules that were cross-linked with Ca2+ and Ba2+ ions and then coated with PLL and alginate. Based on FITC-dextran release these microcapsules have porous wall that enables the rapid contents release. The ARPE-19 cells maintained viability in the Ca2+ and Ba2+ cross-linked microcapsules for at least 110 days. The alginate microcapsules cross-linked with Ca2+ and Ba2+ have sufficiently large pore size for prolonged cell viability and for the release of secreted SEAP model protein (Mw 50 kDa; radius of gyration of 3 nm). ARPE-19 cells show long-term viability and protein secretion within alginate microcapsules cross-linked with Ca2+ and Ba2+. This combination may be useful in cell therapy.

Keywords
Cell encapsulation; Alginate; Protein release; Retinal pigment epithelial cells; ARPE-19
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Alginate-based microencapsulation of retinal pigment epithelial cell line for cell therapy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 7, March 2008, Pages 869–876
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us