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Modification of silk fibroin using diazonium coupling chemistry and the effects on hMSC proliferation and differentiation

Paper ID Volume ID Publish Year Pages File Format Full-Text
9971 656 2008 10 PDF Available
Title
Modification of silk fibroin using diazonium coupling chemistry and the effects on hMSC proliferation and differentiation
Abstract

A simple chemical modification method using diazonium coupling chemistry was developed to tailor the structure and hydrophilicity of silk fibroin protein. The extent of modification using several aniline derivatives was characterized using UV–vis and 1H NMR spectroscopies, and the resulting protein structure was analyzed with ATR-FTIR spectroscopy. Introduction of hydrophobic functional groups facilitated rapid conversion of the protein from a random coil to a β-sheet structure, while addition of hydrophilic groups inhibited this process. hMSCs were grown on these modified silks to assess the biocompatibility of these materials. The hydrophilicity of the silk derivatives was found to affect the growth rate and morphology, but hMSCs were able to attach, proliferate and differentiate into an osteogenic lineage on all of the silk derivatives.

Keywords
Silk fibroin; Chemical modification; Diazonium coupling; Tyrosine; Biomaterials; Mesenchymal stem cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 19, July 2008, Pages 2829–2838
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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